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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?
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"Cloning streams in Node.js's fetch() implementation is harder than it looks. When you clone a request or response body, you're calling tee() - which splits a single stream into two branches that both need to be consumed. If one consumer reads faster than the other, data buffers unbounded in memory waiting for the slow branch. If you don't properly consume both branches, the underlying connection leaks. The coordination required between two readers sharing one source makes it easy to accidentally break the original request or exhaust connection pools. It's a simple API call with complex underlying mechanics that are difficult to get right." - Matteo Collina, Ph.D. - Platformatic Co-Founder & CTO, Node.js Technical Steering Committee Chair
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